Question

1. Complete Table 1 by calculating the volume of 2.0 mg/mL myoglobin, 6.0 M GuHCl, and 0.5 M phosphate buffer needed to make the 15 solutions needed for the experiment.

#	[Myoglobin] (mg/mL)	Myoglobin stock volume (mL)	[GuHCl] (M)	GuHCl stock volume (mL)	Phosphate buffer volume (mL)	Total volume (mL)
1	0.2		0			1000
2	0.2		0.4			1000
3	0.2		0.8			1000
4	0.2		1.0			1000
5	0.2		1.2			1000
6	0.2		1.3			1000
7	0.2		1.4			1000
8	0.2		1.5			1000
9	0.2		1.6			1000
10	0.2		1.7			1000
11	0.2		1.8			1000
12	0.2		2.0			1000
13	0.2		2.2			1000
14	0.2		2.6			1000
15	0.2		3.0			1000

Answer 1