Question

1. Can a heat fixed slide be stored prior to staining?A.Yes B.No

2.When performing a subculture from a broth to a plate you must first perform this step A.sterilize the lip of the broth culture tube B.sterilize the inoculating loop C.resuspend the cells in the broth culture

3.Which contains more cells?A low turbidity broth.B high optical density broth

4. Why might you want to perform a serial dilution of a bacterial sample? A. to identify the organism B.to prepare a sample for staining C.to quantify the number of cells in the original sample D. to dilute out contaminants E.C and D are correct

5. After performing a serial dilution the cultures may be plated as either spread plates or pour plates? A. true B. false

Answer 1

Q1. Option A. Yes.

A sample is is usually heat fixed for preservation, so that no biological processes occur.

Q2. Option B is correct. Sterilized the inoculating loop.

Inoculating loop is the one that are needed to spread the culture. So it must be sterilized to prevent contamination.

Q3. Option B is correct. High optical density broth.

OD value obtained is directly proportional to the cell number.

Q4. Option C is correct. To quantify the number of cells in the original sample.

By multiplying the number of colony counted into number of dilution.

Q5. True. After performing a serial dilution the cultures may be plated as either spread plates or pour plates.