A TLC plate showed two spots with Rf values of 0.25 and 0.26. The plate was removed from the developing chamber, the residual solvent was allowed to evaporate from the plate, and then the plate was returned to the developing chamber. What would you expect to see after the second development was complete?
1.
Which amino acid traveled the furthest up the TLC plate and which
amino acid traveled the least?
2. Why must use a pencil instead of pen to write on TLC plate?
3. What is the function of ninhydrin spray?
4. What are the amino acid present in the unknown
solution?
1. When 2-propanol was used as the develop- ing solvent, two substances moved with the solvent front (Rf = 1) during TLC analysis on a silica gel plate. Can you conclude that they are identical? If not, what additional experiment(s) would you perform?
2.The Rf value of compound A is 0.34 when a TLC plate is developed in hexane and 0.44 when the plate is developed in diethyl ether. Compound B has an Rf value of 0.42 in hexane...
1.
what is the 6th amino acid of human myoglobin?
2. what is the 11th amino acid of human myoglobin?
3. what is the 5th amino acid of insulin?
4. what is thr last amino acid of insulin?
5. what is the 4th amino acid of ATPase?
6. what is the 10th amino acid of ATPase?
7. what is thr last amino acid kf ATPase?
A mutation replaces the original amino acid, leucine, with
threonine in a large protein. The altered protein still functions,
but less efficiently. What amino acid might replace leucine but
with less effect on protein functions? What amino acid might
replace leucine but with even a worse effect on the how the protein
functions?
a) less effect if lysine; worse if alanine
b) less effect if methionine; worse if lysine
c) less effect if methionine; worse if alanine
d) less effect...
“Protein A” is phosphorylated at one amino acid by a kinase,
“protein B”, and dephosphorylated by a phosphatase, “protein
C”.
You have purified all three of these proteins and want to
determine the structural changes that occur when protein A is
phosphorylated or dephosphorylated. Explain in detail how you would
go about designing and conducting this investigation.