In: Biology
3’- T A C G T T C A T A T C C G T A T G T A T A T T – 5’
During translation, ribosomes produce peptide chains with the amino acid sequence as per the triplet codons available on mRNA molecules. So, we will first predict the codons on the mRNA molecule with the help of the base sequence of the given template strand of DNA.
On the mRNA molecule, guanine (G) will take place of the cytosine (C) on the template strand of DNA, and cytosine (C) will take place of guanine (G) on the template strand of the DNA. On mRNA molecule, adenine (A) will take place of thymine (T).on the template strand of DNA, and uracil (U) will take place of adenine (A) on the template strand of DNA.
Please refer to the following table to predict the amino acid sequence for the given template strand of DNA.
DNA template strand | predicted mRNA codons | predicted amino acids |
TAC | AUG | Methionine |
GTT | CAA | Glutamine |
CAT | GUA | Valine |
ATC | UAG | Stop codon |
CGT | GCA | Alanine |
ATG | UAC | Tyrosine |
TAT | AUA | Isoleucine |
ATT | UAA | Stop codon |
The table shows that the ribosome will stop synthesizing polypeptide after the third amino acid as the fourth codon is a stop codon. The predicted amino acid sequence for the given template strand of DNA is Methionine-Glutamine-Valine.