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In: Chemistry

An enzyme was obtained in Dr. Cai’s lab. To study the kinetics of this enzyme, a...

An enzyme was obtained in Dr. Cai’s lab. To study the kinetics of this enzyme, a

graduate student measured the velocity of the reaction at a substrate concentration

of 0.2 mM substrate. He found the initial rate decreased to 1/3 after 1.2 mM

competitive inhibitor was added. KI, the dissociation constant for the inhibitor

binding to the enzyme, is 0.5 mM. What is the KM, the Michaelis constant?

A. 0.33 mM
B. 0.5 mM
C. 0.1 mM

D. 1 mM

E. 10 mM

Solutions

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