Initial rate data for an enzyme that obeys Michaelis-Menten kinetics are shown in the following table....

Initial rate data for an enzyme that obeys Michaelis-Menten kinetics are shown in the following table. When the enzyme concentration is 3 nmol ml−1, a Lineweaver-Burk plot of this data gives a line with a y-intercept of 0.00426 (μmol−1ml s).

[S] μM	v0 (μmol ml−1 s−1)
320	169
160	132
80.0	92.0
40.0	57.2
20.0	32.6
10.0	17.5

a. Calculate Kcat for the reaction

b. Calculate Km for the enzyme

cWhen the reactions in part (B) are repeated in the presence of 12 μM of an uncompetitive inhibitor, the y-intercept of the Lineweaver-Burk plot is 0.352 (μmol−1 ml s). Calculate K′I for this inhibitor.

Expert Solution

queen_honey_blossom answered 8 months ago

1.The following data were obtained in a study of an enzyme known to follow Michaelis-Menten kinetics:...

1.The following data were obtained in a study of an enzyme known to follow Michaelis-Menten kinetics: 2/27/ 20 V0 (mol/min) 217 325 433 488 647 Substrate added (mmol/L) 0.8 2 4 6 1,000 The Km for A) 1 mM., B) 1000mM, C) 2mM, D ) 4mM, E) 6mM this enzyme is approximately: 2. To A) the enzyme concentration. B) the initial velocity of the catalyzed reaction at [S] >> Km. C) the initial velocity of the catalyzed reaction at...

In Michaelis-Menten kinetics, what values would make an enzyme “good” at catalysis?

What are the key differences between the Michaelis-Menten model that describes enzyme kinetics and the Monod...

What are the key differences between the Michaelis-Menten model that describes enzyme kinetics and the Monod model that describes whole-cell or community kinetics?

For enzymes that follow Michaelis–Menten kinetics, the inhibitors that inhibit enzyme activity include reversible inhibitor and...

For enzymes that follow Michaelis–Menten kinetics, the inhibitors that inhibit enzyme activity include reversible inhibitor and irreversible inhibitor. 1. Describe the type of reversible inhibitor. 2. Suggest ways to distinguish between types of inhibitors through enzyme reaction experiments.

The simple Michaelis-Menten model applies only to the initial velocity of an enzyme-catalyzed reaction, that is,...

The simple Michaelis-Menten model applies only to the initial velocity of an enzyme-catalyzed reaction, that is, to the velocity when no appreciable amount of product has accumulated. What feature of the model is consistent with this constraint? Explain.

IV) Many enzymes obey simple Michaelis-Menten kinetics, which are summarized by the equation: rate = (V...

IV) Many enzymes obey simple Michaelis-Menten kinetics, which are summarized by the equation: rate = (V max [S])/([S]+K m ); where V max = maximum velocity, [S] = concentration of substrate; and K m = the Michaelis constant. It is instructive to plug in a few values of [S] into the equation to see how rate is affected. What are the rates for [S] equal to zero, equal to K m , and equal to infinite concentration? Show all work.

When analyses enzyme-kinetics, what can we know with Michaelis-Menten equation? Write down everything you can.

A substrate is decomposed in the presence of an enzyme according to the Michaelis-Menten equation with...

A substrate is decomposed in the presence of an enzyme according to the Michaelis-Menten equation with the following kinetic parameters: Km = 20 g/L Vmax = 12.0 g/L-min (a) Determine the concentration of substrate after leaving the second reactor in a two-reactor series of 20-liter CSTRs. The flow rate is 2.00 L/min. The inlet substrate concentration is 40 g/L. The enzyme concentration in the two reactors is maintained at the same value all of the time. (b) Determine the conversion...

Draw a Michaelis Menten graph to represent normal pyruvate carboxylase kinetics in the conversion of pyruvate...

Draw a Michaelis Menten graph to represent normal pyruvate carboxylase kinetics in the conversion of pyruvate to oxaloacetate. Explain why you drew things as you did.

Explain the meaning of the three assumptions for the Michaelis-Menten kinetics. Express each assumption in mathematical...

Explain the meaning of the three assumptions for the Michaelis-Menten kinetics. Express each assumption in mathematical terms.

Question