In: Chemistry
Initial rate data for an enzyme that obeys Michaelis-Menten kinetics are shown in the following table. When the enzyme concentration is 3 nmol ml−1, a Lineweaver-Burk plot of this data gives a line with a y-intercept of 0.00426 (μmol−1ml s).
[S] μM | v0 (μmol ml−1 s−1) |
320 | 169 |
160 | 132 |
80.0 | 92.0 |
40.0 | 57.2 |
20.0 | 32.6 |
10.0 | 17.5 |
a. Calculate Kcat for the reaction
b. Calculate Km for the enzyme
cWhen the reactions in part (B) are repeated in the presence of 12 μM of an uncompetitive inhibitor, the y-intercept of the Lineweaver-Burk plot is 0.352 (μmol−1 ml s). Calculate K′I for this inhibitor.