Question

In: Chemistry

Using 1.0 nM enzyme X for the enzyme kinetics experiments, you obtained the reaction rates at...

Using 1.0 nM enzyme X for the enzyme kinetics experiments, you obtained the reaction rates at different concentrations of substrate (shown in the following table). Assume that the enzyme X follows a typical Michaelis-Menten kinetics.

[substrate] (μM) Reaction rate (μmol/s)

2 7

5 17

10 29

20 44

50 67

100 80

200 89

500 95

1000 98

a) Estimate the Vmax and Km values of the enzyme X in this reaction using the double reciprocal plot.

b) If 5.0 nM of the enzyme X is used in the experiment, what will be the Vmax and Km values of the enzyme X?

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Expert Solution

queen_honey_blossom answered 5 months ago
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