List the sequence of structures that a single amino acid
(initially in a protein molecule that you eat) goes through from
the moment it passes your lips to when it ends up in a hepatocyte.
Then explain what (if any) chemicals relevant to digestive
physiology it is exposed to at each step and what happens to the
protein and eventually protein fragments each step of the way.
1.
Which amino acid traveled the furthest up the TLC plate and which
amino acid traveled the least?
2. Why must use a pencil instead of pen to write on TLC plate?
3. What is the function of ninhydrin spray?
4. What are the amino acid present in the unknown
solution?
\Match the following descriptions with the corresponding amino
acids:
This amino acid is responsible for the strength of rhino horns
and wood lignin due to the strong hydrophobic interactions between
them.
Subtle decreases in the serum pH lead to the protonation of this
amino acid, which promotes O2 release from hemoglobin.
This amino acid is the nucleophile chymotrypsin uses to attack
peptide bonds.
This amino acid disrupts α-helices because it makes the nearby
protein backbone too flexible (and prevents proper...
Indicate which of the amino acid residues in the following
peptide sequence contains a group that has a negative charge for
its most likely charge state at pH 4.
Met-Tyr-Ile-Trp-Gln-Val-Cys-Pro-Lys
which of the following statements about the charge distribution
on the amino acid glutamate is incorrect ?
glutamate has three different ionizable groups
at a very low pH, the most abundant form of glutamate will have
net charge of +1
at a very high pH the most abundant form of glutamate will have
a net charge of -2
at a pH of aprox. 3.2 glutamate will not contain any charged
groups
all of the above are correct
Shown following is the first 30 amino acid (one-letter code)
region of this putative amino acid sequence for p18:
1YFNPS
6TSDWPT 11LAPAN 16YTFLF 21FLARY 26WYINL30
A) Based on the partial amino acid sequence provided, do you
think that it is more likely that this region of p18 associates
with a cellular membrane or is exposed to the cytosol?
B) Why or why not?
C) Describe how you would experimentally demonstrate if p18 was
associated with the membrane or the cytosol.