what would be different for bacterial cells that happened to be inside the agar and those...

what would be different for bacterial cells that happened to be inside the agar and those that were on top of the agar in plates from the pour plate method? explain.

Expert Solution

Bacterial cells that are inside the agar are

Obligate anaerobe - which cannot survive in the normal atmospheric concentrations of oxygen

Facultative anaerobe - which can grow with or without oxygen

Aerotolerant organisms - which do not require oxygen as they metabolise energy anaerobically.

Because the environment inside the agar is free from oxygen so anaerobic bacteria which doesn’t require oxygen for its growth will grow in it whereas aerobic bacteria which requires oxygen for its growth can’t grow inside the agar.

Bacterial cells that are present on the surface of the agar are

Obligate aerobes - which requires oxygen and can survive in the highest concentration of oxygen

Microaerophiles - which requires oxygen and can survive only in lesser concentration of oxygen for its growth.

Hence the anaerobic bacteria which survives only in an anaerobic environment cannot grow on the top of the agar

gladiator answered 1 year ago

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