Question

how to increase the hydrolysis ability of enzyme?

Answer 1

In its simplest definition, hydrolysis is a chemical reaction in which water is used to break down the bonds of a particular substance. In biotechnology and as far as living organisms are concerned, these substances are often polymers.

There are three main types of hydrolysis: salt, acid, and base hydrolysis.

Hydrolysis reactions in living organisms are performed with the help of catalysis by a class of enzymes known as hydrolases. The biochemical reactions that break down polymers, such as proteins (which are peptide bonds between amino acids), nucleotides, complex sugars and starch, and fats are catalyzed by this class of enzymes. Within this class are lipases, amylases, proteinases hydrolyze fats, sugars, and proteins, respectively.

A catalyst is a chemical that increases the rate of a chemical reaction without itself being changed by the reaction. The fact that they aren't changed by participating in a reaction distinguishes catalysts from substrates, which are the reactants on which catalysts work. Enzymes catalyze biochemical reactions. So as we know that enzymes are also catalyze so for that there are a specific condition for the best performance . There are some factors -

I) Enzymes are specific - At a specific condition they shows their maximum ability.

II)Enzymes work under mild conditions - At the proper condition they work maximum.

III)Enzymes are stereospecific - Here also they have a specific work field.

IV) Enzymes are macromolecules - The macromolecules are composed of protein, or in a few cases, RNA. Most chemical catalysts are either surfaces, for example, metals like platinum, or else small ions, such as hydroxide ions. V) Enzymes are often regulated- The regulation occurs either by the concentration of substrates, by binding small molecules or other proteins, or by covalent modification of the enzymes' amino acid side chains. Thus, an enzyme's effectiveness can be altered without changing the concentration of the enzyme; on the other hand, the effectiveness of a chemical catalyst is generally determined by its overall concentration.

If we provide the appropriate and specific condition for the specific enzyme then we get the maximum output.

Thanking you. Hope you will get help from the answer.