In: Biology
A group of bird embryos was studied to see if the hair cells in cochlea were able to regenerate after trauma. The first group of bird embryos was treated with growth factors similar to brain-derived neurotrophic factor molecules. The second group of bird embryos had specialized growth genes introduced into their genomes through a virus. The third group was untreated and left to develop without growth factors and gene therapy. The total number of hair cells per cochlea was determined at 10 days and compared at hatching (day 21) through scanning electron microscopy.
What is the manipulated, responding, and controlled variable in the study?