Consider the mitochondrial electron transport chain that utilizes NADH a. Write a NET balanced equation for...

Consider the mitochondrial electron transport chain that utilizes NADH

a. Write a NET balanced equation for the entire reaction run by Complex I, Complex III and Complex IV together. Include the protons pumped from the inside to the outside of the matrix.

b. If you assume that the membrane potential is 200 mV and the pH gradient is 0.5 and the temperature is 37 C, how much energy is stored by the total reaction you wrote down in (a) as a membrane proton motive force per mole of NADH consumed?

c. Now write down the two half reactions that make up the reaction from part (a) (ignoring the protons pumped).

d. Using the values for E0 in the book for these reactions, what is the standard energy of the reaction you wrote down in (a)? e. What fraction of the total oxidation energy of NADH is stored as ATP. Assume that the reaction ADP + Pi à ATP + H2O has a free energy of 50 kJ/mole and assume the actually free energy of NADH oxidation is the same as the standard free energy you calculated above.

Please show work for a-d thank you.

Solutions

Expert Solution

C. Complex 1: NADH-CoQ oxidoreductase

Net reaction: NADH + H⁺ + CoQ ---> NAD+ + CoQH₂

Complex I pumps protons outside the mitochondria.

ATP is produced

Complex II: Succinate-CoQ reductase

Net reaction: Succinate + CoQ --> Fumarate + CoQH₂

Conversion of succinate to fumarate is reaction of TCA cycle and is catalyzed by succinate dehydrogenase.

Not a proton pump.

Complex III: CoQH₂-cytochrome c oxidoreductase

Net reaction: CoQH₂ + 2 cyt c [Fe (III)] ---> CoQ + 2 cyt c [Fe (II)] + 2 H⁺

Complex III pumps protons outside the mitochondria.

ATP produced

Complex IV: cytochrome oxidase

Net reaction: 2 cyt c [Fe (II)] + 1/2 O₂ + 2 H+ ---> 2 cyt c [Fe (III)] + H₂O

Complex IV pumps protons outside the mitochondria.

ATP produced

gladiator answered 1 year ago

Next > < Previous

Related Solutions

Describe the process of electron transport provided by NADH and FADH2 through the electron transport chain

1. Describe mitochondrial electron transport from NADH to O2 by listing the protein complexes involved, the...

1. Describe mitochondrial electron transport from NADH to O2 by listing the protein complexes involved, the mobile electron carriers, at which point protons are transported, and how many protons at each step. 2. Based on your knowledge of the structure of protein Complex I of the ETC, describe how electron transport is linked to the passage of 4 protons through Complex I. Describe the protein structure changes that occur. 3. . Describe the subunit structure ATP Synthase. What subunits comprise...

Describe how NADH and FADH2 are converted into ATP by the electron transport chain and ATP...

Describe how NADH and FADH2 are converted into ATP by the electron transport chain and ATP synthase. Describe the proteins involved, and describe the roles of H+, e-, and O2 in the process.

1. Compare and contrast the point of entry for NADH-derived electrons into the electron-transport chain on...

1. Compare and contrast the point of entry for NADH-derived electrons into the electron-transport chain on the matrix side vs the cytosolic side of the inner mitochondrial membrane. Which of these entry points provides the most energy? Why?

From what reactions is NADH made in complex I in electron transport chain? In Succinate -->...

From what reactions is NADH made in complex I in electron transport chain? In Succinate --> Fumarate in Complex II what reaction is this, and in which pathway?

how reduced NADH and FADH2 feed into the electron transport chain to drop off electrons and...

how reduced NADH and FADH2 feed into the electron transport chain to drop off electrons and H+.

1. Which of components of the electron transport chain directly move protons across the inner mitochondrial...

1. Which of components of the electron transport chain directly move protons across the inner mitochondrial membrane? 2. Consider fermentation. How much ATP is generated during fermentation? How does the amount of ATP generated by fermentation compare to aerobic respiration? In humans, why can't fermentation sustain life? (Hint: Think of two reasons—one is related to the product of fermentation and what happens if it accumulates.) 3. Given this segment of a double-stranded DNA molecule, draw the two major steps involved...

(g) Within the electron transport chain, complex ____ represents the entry port for electrons from NADH...

(g) Within the electron transport chain, complex ____ represents the entry port for electrons from NADH while complex ____ represents the entry point for electrons from FADH2. Group of answer choices I; III II; III I; II II; IV III; IV (h) You have a solution that contains the pyruvate dehydrogenase complex and all of the enzymes of the citric acid cycle, but none of the intermediates of the citric acid. If you add 3.0 mM each of pyruvate, coenzyme...

Assuming the electron transport chain received electrons from NADH and FADH2 in a 3:1 ratio as...

Assuming the electron transport chain received electrons from NADH and FADH2 in a 3:1 ratio as the wild type for a particular organism, what would the consequences be of a change to a 1:1 ratio? Select all that apply.

In mitochondrial electron transport, what is the direct role of O2?

The role of O2 in electron transport In mitochondrial electron transport, what is the direct role of O2? a)to provide the driving force for the synthesis of ATP from ADP and Pi b)to function as the final electron acceptor in the electron transport chain c)to oxidize NADH and FADH2 from glycolysis, acetyl CoA formation, and the citric acid cycle d)to provide the driving force for the production of a proton gradient

Subjects