Question

In: Biology

Consider the mitochondrial electron transport chain that utilizes NADH a. Write a NET balanced equation for...

Consider the mitochondrial electron transport chain that utilizes NADH

a. Write a NET balanced equation for the entire reaction run by Complex I, Complex III and Complex IV together. Include the protons pumped from the inside to the outside of the matrix.

b. If you assume that the membrane potential is 200 mV and the pH gradient is 0.5 and the temperature is 37 C, how much energy is stored by the total reaction you wrote down in (a) as a membrane proton motive force per mole of NADH consumed?

c. Now write down the two half reactions that make up the reaction from part (a) (ignoring the protons pumped).

d. Using the values for E0 in the book for these reactions, what is the standard energy of the reaction you wrote down in (a)? e. What fraction of the total oxidation energy of NADH is stored as ATP. Assume that the reaction ADP + Pi à ATP + H2O has a free energy of 50 kJ/mole and assume the actually free energy of NADH oxidation is the same as the standard free energy you calculated above.

Please show work for a-d thank you.

Expert Solution

A.

C. Complex 1: NADH-CoQ oxidoreductase

Net reaction: NADH + H⁺ + CoQ ---> NAD+ + CoQH₂

Complex I pumps protons outside the mitochondria.

ATP is produced

Complex II: Succinate-CoQ reductase

Net reaction: Succinate + CoQ --> Fumarate + CoQH₂

Conversion of succinate to fumarate is reaction of TCA cycle and is catalyzed by succinate dehydrogenase.

Not a proton pump.

Complex III: CoQH₂-cytochrome c oxidoreductase

Net reaction: CoQH₂ + 2 cyt c [Fe (III)] ---> CoQ + 2 cyt c [Fe (II)] + 2 H⁺

Complex III pumps protons outside the mitochondria.

ATP produced

Complex IV: cytochrome oxidase

Net reaction: 2 cyt c [Fe (II)] + 1/2 O₂ + 2 H+ ---> 2 cyt c [Fe (III)] + H₂O

Complex IV pumps protons outside the mitochondria.

ATP produced

gladiator answered 1 year ago

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