Question

1. Select a contrast mode in microscopy. In the space below, describe and illustrate it in detail (hint: phase, dark-field, bright-field) (1 page limit)

Answer 1

Brightfield, darkfield, and phase contrast are the most common label-free contrast modes used in optical microscopy.Brightfield imaging is most suitable for observing samples with strong absorption. Darkfield imaging provides good contrast for subresolution features, since it only captures high-angle scattered light. Phase contrast is used for unstained and transparent biological samples, allowing visualization of shape and density variations.

In optical microscopy, dark-field describes an illumination technique is used to enhance the contrast in unstained samples. It works by illuminating the sample with light that will not be collected by the objective lens and thus will not form part of the image.

Brightfield, darkfield, and phase contrast are the most common label free contrast modes used in optical microscopy. Brightfield imaging is most suitable for observing samples with strong absorption. Darkfield imaging provides good contrast for subresolution features. It only captures high-angle scattered light.

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