Question

In: Biology

Propose an experiment for localizing a protein (of your choice) to an alternative cellular compartment. Then,...

Propose an experiment for localizing a protein (of your choice) to an alternative cellular compartment. Then, speculate what characteristics/symptoms this would create on a cellular, tissue/organ, and organismal level.

Solutions

Expert Solution

Transport of proteins to different cellular compartments is mediated by the secretory pathway depending upon the nature of the sequence tag. Different cell organelles exhibit a characteristic organellar localization signal peptide sequence (tag) which can be located at the N' or C' termini.
Examples:
ER retention signal = KDEL
Peroxisome localization signal = S-K-L
Nuclear localization signal = PPKKKRKV

If we want to mislocalize a specific protein to another cell organelle, we can do it so by removing its native tag and adding other organellar specific tags.
Example: p53 is tumor suppressor gene. It is a transcription factor. So, it is localized in the nucleus. If we remove nuclear localization signal from it and add peroxisomal localization signal, it gets localized to the peroxisome.
Since p53 is sequestered from the nucleus where it has the function, the cell experiences drastic effects.
Since it is a tumor suppressor gene,
The cell undergoes continuous cell division.
The tissue becomes cancerous
The organism eventually dies due to metastasis.


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