Question

Microorganisms called psychrophiles will grow only at low temperatures (at or near 0°C). This restriction on a microbes growth would most directly impact

a. inoculation

b incubation

c isolation

d inspection

e inspection

f information gathering

g identification

Answer 1

Ans. This would directly impact- inoculation, isolation, information gathering

Restriction on growth of a microbes at very low temperature (psychrophiles) would impact on inoculation. Inoculation involves the transfer of the microbes to another sytem (animal body or plant body) to analyse the effect of the microbes (diseases and disorders) on plants or animals. Animals have a body temperature (for example human has normal body temperature of 37^oC) and hence microbes will not be able to grow. If it does not grow analysis of effect of the microbes is not possible. Isolation from the culture medium and use at room temperature will not be possible for these microbes and hence such microbes needs to be handled at this very low temperature always which ia a very difficult task and nearly impossible. Information gathering for such microbes including proteins and enzymes, many other biomolecules and chemicals which are heat sensitive will be very difficult because these microbes' proteins and enzymes will lose its 3-D strucre at temperature outside its low temperature growth chamber and hence analysis of structure of proteins and enzymes at room temperature is not possible. Hence, information gathering needs to be performed for many chemicals at low tempratute and therefore becomes a very difficult task.