What does the dye intensity tell you? What information do you get about the fold change?...

What does the dye intensity tell you? What information do you get about the fold change? Why is it important to look at both types of data when considering if a gene might be important for the process you want to study?

Expert Solution

For studying particular process, it is important to know how the genes related to that process is expressed and in which amount it is expressed. If it is under or over expressed, then the process is down regulated. Real time PCR is an important technique in studying gene expression. It will gives data regarding how much fold of gene is expressed, with respect to the intensity of dye. Here the fold indicates the quantity from initial to final expression of gene. The dye is absorb by DNA and it is measured by a filter. Here we are studying gene expression hence our primer set and other PCR conditions are set to amplify particular gene. The amount of dye is absorbed in DNA is directly proportional to the fold of gene. Higher intensity of absorbed DNA signals means higher fold of gene expressed and vice versa.

Higher intensity of dye absorb by DNA means higher gene expression and the number of fold is increased. if the observed gene expression is in normal required range of particular process than that particular gene might be important for the process.

gladiator answered 1 year ago

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