can you uncouple generation of proton gradient in mitochondria from ATP synthesis?how? what is the cellular...

can you uncouple generation of proton gradient in mitochondria from ATP synthesis?how? what is the cellular consequence of this?

Expert Solution

Yes, we can uncouple the generation of proton gradient in mitochondria from ATP synthesis by using specific molecules called as uncouplers. These molecules uncouple the two processes and the example includesCarbonyl cyanide p-trifluoro-methoxyphenyl hydrazone (FCCP) and carbonylcyanide-3-chlorophenylhydrazone (CCCP).

If we use any uncoupling reagent in mitochondria, then ATP synthesis and generation of proton gradient will not take place together. Without Proton gradient, there will be no source of electrochemical energy which can be used by ATP synthase enzyme to synthesise ATP by phosphorylation of ADP into ATP.

Under normal condition, Proton gradient is generated across in the mitochondrial membrane during electron transport chain. The electrochemical energy stored in Proton gradient is used by ATP synthesis enzyme for the synthesis of ATP. This is called as oxidative phosphorylation or chemiosmosis.

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gladiator answered 2 years ago

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