Question

An experiment is done to measure electron transport and ATP synthesis in isolated mitochondria. Sketch a plot of the hypothetical experimental results if you measure both O2 consumption and ATP synthesis ( Left Y-axis for O2 consumption; Right Y-axis for ATP production, overlay both on the same plot. You may use different color or simply indicate the curves) versus time (X-axis). Start the experiment (t = 0) with succinate, ADP, and Pi in the mix and successively add DNP at t = 4 min., and then Oligomycin (at t = 8 min.). Indicate the point in the plot that each agent is added. (4 points)

Answer 1

Given data is,

Q) An experiment is done to measure electron transport and ATP synthesis in isolated mitochondria. Sketch a plot of the hypothetical experimental results if you measure both O2 consumption and ATP synthesis ( Left Y-axis for O2 consumption; Right Y-axis for ATP production, overlay both on the same plot. You may use different color or simply indicate the curves) versus time (X-axis). Start the experiment (t = 0) with succinate, ADP, and Pi in the mix and successively add DNP at t = 4 min., and then Oligomycin (at t = 8 min.). Indicate the point in the plot that each agent is added. (4 points).

The answer is,

--> ATP union - substrate level phosphorylation energy connections (by the aadition of an uncoupler of ATP union and electron transport at time 4 minutes); and afterward an inhibitor of ATP synthase (at t = 8 min.)

Then the coupling follows micahel-menton's energy related after responses

Succinate [R] + ADP + iP (inorganic phosphate) (L) < - > Free substrate focus [R] + ATP [RL]

α= [RL]/[R]+[RL]; where α is considered as Hill coefficient which is additionally speaks to list of cooperativity forever its worth lies between 0 to 1.

progressively including an uncoupler of ATP union and electron transport (at t = 4 min.) and afterward include an inhibitor of ATP synthase (at t = 8 min.)- - > α = < 1 - > negative cooperativity - > 1/(number of restricting locales) with less restricting destinations. Low ATP creation.

gives scatchard plots as follows ATP union versus O2 utilization.