Question

Lactate dehydrogenase catalyzes the conversion of pyruvate into lactate. kinetics

experiment was done to test the effect of the inhibitor oxamate on the ability of

lactate dehydrogenase to convert pyruvate into lactate. The data with and without

inhibitor was plotted as a Lineweaver-Burk plot. The velocity is in units of umol/min

and the concentration of pyruvate is in units of MM

The equation of the line of best fit for the reaction without oxamate is: y 3.1x +7.4

The equation of the line of best fit for the reaction with oxamate is: y = 5.4x+29

What are the values of KM. Vmax, KMaPP and Vmax aPP? What type of inhibitor is oxamate?

Please show all your calculations. Round your final answers to the nearest 12th and include

the correct units.

that the entire question

Answer 1

In an LB plot, we have 1/[S] on the X-axis and 1/V on the y-axis. We the resultant is a straight line represented by (where 'y' is the y coordinate of a given point; 'm' is the slope, 'x' is the x coordinate of the point and 'c' is the constant.

Km is the substrate concentration when the velocity of the reaction is half of its maximum value while Vm is the maximum velocity of the reaction.
The former is the reciprocal of the X-intercept while the latter is the reciprocal of the Y-intercept on the line of best fit without oxamate.

The equation for line for without oxamate situation is given as:

To obtain the Km (reciprocal of x-intercept),

Hence, Km = 0.42MM

To obtain Vm (reciprocal of y-intercept)

Hence, Vm = 0.14

The equation for with oxamate situation is given by

To obtain Km^app ( reciprocal of x-intercept)

Hence, Km = 0.19 MM

To obtain Vm^app (reciprocal of y-intercept),

Hence, Vm^app= 0.03