Question

Draw a Michaelis Menten graph to represent normal pyruvate carboxylase kinetics in the conversion of pyruvate to oxaloacetate. Explain why you drew things as you did.

Answer 1

Michael is menten equation describes the relationship between the concentration of substrate and velocity of an enzyme catalysed reaction. The graph which I have drawn shows pyruvate as the substrate and enzyme is pyruvate carboxylase. It shows that as the concentration of pyruvate increases the rate of reaction also increases. The rate of reaction is highest at a point which we call as maximum velocity. After achieving maximum velocity, the reaction achieves a plateau. It is because now the number of enzyme molecules, that is, the molecules of pyruvate carboxylase has become limited or the number of substrate molecules, pyruvate molecules have become Limited. All the enzyme active sites are occupied by pyruvate molecules. For the increase in the rate of reaction will be seen only if the concentration of enzyme and substrate is increased.

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