The commercially available pUC57 plasmid is circular with 2710 bp. It has one EcoRI cleavage site...

The commercially available pUC57 plasmid is circular with 2710 bp. It has one EcoRI cleavage site at 396 and one HindIII cleavage site at 471. How many bands would you expect to see on the gel if both enzymes were used to digest the enzyme and the digestion was complete? Explain your answer.

Using the same plasmid and restriction enzymes as in question 2, how many bands would you expect to see on the gel if the digestion were partial? A partial digestion means that

not every site on every molecule is cleaved by the restriction enzyme. Explain your answer

Expert Solution

First Question:

As the plasmid is circular & it has two restriction sites (EcoRI & HindIII), after digesting the plasmid using both restriction enzyme if complete digestion occurs, we will observe two bands. This is due to the fact that we will get two fragments- one between 396 & 471 bp (Site A; 75 bp) & another between 471 & 396 bp (Site B; 2635 bp). This is shown in below picture.

Second Question:

When partial digestion occurs, we will expect to observe one band. This is due to the fact that in partial digestion only one of the restriction sites will cleave & other will not cleave. It will be resulted in the one band of 2710 bp (Total plasmid size).

gladiator answered 2 years ago

A 3000 bp circular DNA was treated with both HindIII and EcoRI restriction enzymes. EcoRI cuts...

A 3000 bp circular DNA was treated with both HindIII and EcoRI restriction enzymes. EcoRI cuts at position 100. HindIII cuts at positions 500 and 2000. Which of the following represents the fragment that would be observed if the restricted DNA were analyzed using agarose gel electrophoresis? A. 400, 100, 1000, 1500 B. 400, 500, 1500 C. 400, 100, 1600 D. 500, 100, 500, 900 E. 500, 1000, 600

1.Consider a plasmid (circular DNA) which has 1000 bp and a G+C content of 50%. How...

1.Consider a plasmid (circular DNA) which has 1000 bp and a G+C content of 50%. How many phosphorous atoms are there? 2. Consider a plasmid (circular DNA) which has 1000 bp and a G+C content of 50%. How many nitrogen atoms are there? 3. Suppose the G-C content in the plasmid (1000 bp) was GREATER than 50%. Which (if any) of your above answers would change, and how? (increase/decrease/no change?) a) # of phosphodiester bonds b) # of H-bonds c)...

8. A circular plasmid of 6200 base pairs (bp) with three restriction enzyme sites at 900,...

8. A circular plasmid of 6200 base pairs (bp) with three restriction enzyme sites at 900, 1300, and 4000 bp. You digest this plasmid, then run the digest on a gel. What are the expected DNA fragment sizes? 9. A linear plasmid of 6200 base pairs (bp) with three restriction enzyme sites at 900, 1300, and 4000 bp. You digest this plasmid, then run the digest on a gel. What are the expected DNA fragment sizes? 10. In a random...

If a circular plasmid is cut one with a restriction enzyme what is the result? how...

If a circular plasmid is cut one with a restriction enzyme what is the result? how do we determine how many times a restriction enzyme cut.

Create a table on one page of your site that has at least one colspan or...

Create a table on one page of your site that has at least one colspan or rowspan. Style it with at least three selectors in your stylesheet. You can use element, class and/or ID. (Note: do not use deprecated HTML attributes - use CSS!) Add at least two font stacks to your website including one web font from Google Fonts (Links to an external site.) Use padding, margin, and border at least once each in your stylesheet.

The firm referred to in problem #1 has 3 projects available: One with a cost of...

The firm referred to in problem #1 has 3 projects available: One with a cost of $4000 and an IRR of 18%; one with a cost of $3000 and an IRR of 20%; and one with a cost of $6000 and an IRR of 6%. Do the following: Compute the optimal capital budget. In other words, how much capital must the firm raise in order to invest in all projects whose IRR exceeds the WACC? What projects should be accepted?

Fox Industries Co (Fox) manufactures engineering parts. It has one operating site and a customer base...

Fox Industries Co (Fox) manufactures engineering parts. It has one operating site and a customer base spread across Europe. The company’s year-end was 30 April 20X3. Below is a description of the purchasing and payments system. Purchasing system: Whenever production materials are required, the relevant department sends a requisition form to the ordering department. An order clerk raises a purchase order and contacts a number of suppliers to see which can dispatch the goods first. This supplier is then chosen....

Fred has the following data r e garding an available for sale debt security: YEAR ONE...

Fred has the following data r e garding an available for sale debt security: YEAR ONE PURCHASE PRICE 2,000 12/31 YEAR ONE FAIR MARKET VALUE 3,000 YEAR TWO SALES PRICE 5,000 What is the amount of debit entry to OCI when instrument is sold in year 2? What is the amount of recognized gain on income statement when instrument is sold in year 2?

ELT Corporation, which makes only one product, Yester, has the following information available for the coming...

ELT Corporation, which makes only one product, Yester, has the following information available for the coming year. ELT expects sales to be 27,000 units at $32 per unit. The current inventory of Yester is 3,500 units. ELT wants an ending inventory of 4,200 units. Each unit of Yester takes two units of component K. Component K is estimated to cost $9 per unit. Current inventory of K is 3,900 units. ELT wants 5,800 units of K on hand at the...

Question