Question

8. In RFLP, this method can be used to transfer DNA to a filter so that it can be detected with a probe: a. Hybridization b. VNTR c. PCR d. Southern Blotting

9. In VNTR analysis, this consists of a complex barcode-like band pattern generated from multiple VNTR loci: a. Single-locus probe b. Multi-locus probe c. Southern Blotting d. None of the above

10. This technique used in VNTR analysis was pioneered by Sir Alec Jeffreys in 1984: a. Single-locus probe b. Multi-locus probe c. Southern Blotting d. PCR

11. Multi-locus probe approaches have been very successful in which of the following cases: a. A cold case with degraded DNA b. A rape with a very small DNA sample c. A dispute over parentage d. A robbery

12. Single-locus probes generate a simple pattern called a: a. DNA Profile b. Genetic Fingerprint c. DNA Fingerprint d. All of the above

13. The first case to utilize DNA evidence in a criminal investigation occurred in the United Kingdom in what year: a. 1990 b. 1983 c. 1980 d. 1992

14. In VNTR analysis, the bands of similar size are grouped into what: a. Bins b. Loci c. Alleles d. Profiles

Answer 1

8. Southern blot combines transfer of electrophoresis-parted  DNA fragments to a filter membrane and successive fragment detection by probe hybridization.

Correct answer is "southern blotting"

9 and 10: In 1984, Multi locus probe was pioneered by Sir Alec Jeffreys. Multi locus probe produces a complex barcode-like band pattern generated from multiple VNTR loci in VNTR analysis

Correct answer for both 9 and 10 is "multi-locus probe"

12: Single-locus probes generate a simple pattern called a DNA Profile .

Correct answer is "DNA profile"

14: In VNTR anlysis, the Bands of similar size aregrouped into bins, generally the width of this bin is about 10% of the mean size of the VNTR segment at the center of the bin.

Correct answer is "bins"