Question

1. The assumptions made in calculating the Michaelis-Menten Equation include

Question options:

a)	that the value of k-2 can be ignored.
b)	that the concentration of the substrate is greater than the concentration of E.
c)	that the formation and decomposition of ES is the same for a period of time.
d)	A, B and C
e)	A and B only

2. Which of the following pairings regarding cofactors is correct?

Question options:

a)	NADH and NADPH: contain isoalloxazine component
b)	FAD and FMN: decarboxylation reactions
c)	coenzyme A: contains a pyrimidine
d)	biotin: associates with the enzyme through an acidic R group lining the active site pocket
e)	None of the above is correct.

3. Which of the following statements is false?

Question options:

a)	ATP is often coupled to phosphoryl group transfer reactions.
b)	Lipoamide functions as a swinging arm that carries acyl groups between active sites in multienzyme complexes.
c)	Tetrahydrofolate is produced through several reduction steps of folate which require FADH2 reducing equivalents.
d)	Beriberi is the result of vitamin C deficiency.
e)	A and B are false.
f)	C and D are false.
g)	None of the above (a-d) is false.

4. In solution α-D-glucopyranose and β-D-glucopyranose ________.

Question options:

a)	rapidly polymerize to form a heteropolymer
b)	form a racemic mixture
c)	form an equilibrium mixture
d)	can never exist together

5. Which statement is false about the type of sugar unit in DNA?

Question options:

a)	It is an epimer of glucose.
b)	It is a deoxy form of ribose
c)	It has a D-configuration
d)	It is cyclic in DNA.
e)	More than one of the above statements is false regarding the sugar units in DNA.

Answer 1

1. Michaelis Menten mechanism is given for enzyme catalysis. In the mechanism, some assumptions are made. The steady state approximation is done in the mechanism, which means that rate of formation of intermediate [ES] is same as that of rate of its decomposition. So, option C is correct. Enzyme is used in very less concentration as compared to substrate because enzyme is a catalyst here. So, option b is also correct. ther k-2 is ignored in the reaction because rate of decomposition is very slow process, so it is rate determining step. So, option a is also correct. Thus option d is the correct answer.

2. option a is incorrect because  isoalloxazine ring is a component of FAD and FADH2. OPTION B is also incorrect because TPP catalyses decarboxylation reaction. option c is correct as it contains a pyrimidine ring which is a part of adenine. So, correct option is c.

3. ATP is often coupled to phosphoryl group transfer reactions. as it has higher potential for transfer. So, option a is correct. option b is also correct. beriberi is caused by the deficiency of Vitamin B1. Scurvy is caused by vitamin c deficiency. So, option a and b are correct and c and d are false. Correct option is f.

4. They form an equilibrium mixture. They exist in open chair form at equilibrium. They both are anomers of each other. SO, option c is correct.

5. Option a is false because sugar unit in DNA is not an epimer of glucose. However, Galactose is an epimer of glucose. It is a deoxy form of ribose as the full ame of DNA is deoxyribose nucleic acid. So, option b is correct. DNA has a D-configuration and it is cyclic in DNA. So, option a is correct option.