1. Starting from the enzyme-catalyzed reaction: S -> P Derive the (a) Michaelis-Menten Equation (b) starting...

1. Starting from the enzyme-catalyzed reaction:

S -> P

Derive the (a) Michaelis-Menten Equation (b) starting from the Michaelis-Menten equation, derive the Lineweaver-Burker plot. Provide brief explanation in each step.

2. Predict the optimum pH and temperature for human saliat amylase. Why did you arrive on the prediction?

Expert Solution

queen_honey_blossom answered 1 year ago

The simple Michaelis-Menten model applies only to the initial velocity of an enzyme-catalyzed reaction, that is,...

The simple Michaelis-Menten model applies only to the initial velocity of an enzyme-catalyzed reaction, that is, to the velocity when no appreciable amount of product has accumulated. What feature of the model is consistent with this constraint? Explain.

A substrate is decomposed in the presence of an enzyme according to the Michaelis-Menten equation with...

A substrate is decomposed in the presence of an enzyme according to the Michaelis-Menten equation with the following kinetic parameters: Km = 20 g/L Vmax = 12.0 g/L-min (a) Determine the concentration of substrate after leaving the second reactor in a two-reactor series of 20-liter CSTRs. The flow rate is 2.00 L/min. The inlet substrate concentration is 40 g/L. The enzyme concentration in the two reactors is maintained at the same value all of the time. (b) Determine the conversion...

Using the Michaelis-Menten equation, derive an expression that will determine Km as a function or in...

Using the Michaelis-Menten equation, derive an expression that will determine Km as a function or in terms of Vmax, V0 and [S]. With this derived equation then calculate: a) Km B) Indicate at each substrate concnetration whether this Km changes with the changing S C) Using the double reciprocal plot, determine Km.

For the reaction E+S > ES> P, the michaelis menten constant, Km, is actually a summary...

For the reaction E+S > ES> P, the michaelis menten constant, Km, is actually a summary of three terms. What are they? How is Km determined graphically?

1. If the enzyme-catalyzed reaction E + S ↔ ES ↔ E + P takes place...

1. If the enzyme-catalyzed reaction E + S ↔ ES ↔ E + P takes place near Vmax to the enzyme (E), what can be concluded about the relative concentrations of S and ES? a) High [S], [ES] is low b) High [S], [ES] are at their maximum c) Low [S], [ES] is low d) Low [S], [ES] is at its maximum 2. Which of the following statements about Michaelis-Menten kinetics is correct? a) Km = the substrate concentration required...

Derive the kinetic equations (Michaelis-Menten equation) about 3 reversible inhibition kinetics: competitive inhibition, uncompetitive equation, and...

Derive the kinetic equations (Michaelis-Menten equation) about 3 reversible inhibition kinetics: competitive inhibition, uncompetitive equation, and noncompetitive equation.

1.The following data were obtained in a study of an enzyme known to follow Michaelis-Menten kinetics:...

1.The following data were obtained in a study of an enzyme known to follow Michaelis-Menten kinetics: 2/27/ 20 V0 (mol/min) 217 325 433 488 647 Substrate added (mmol/L) 0.8 2 4 6 1,000 The Km for A) 1 mM., B) 1000mM, C) 2mM, D ) 4mM, E) 6mM this enzyme is approximately: 2. To A) the enzyme concentration. B) the initial velocity of the catalyzed reaction at [S] >> Km. C) the initial velocity of the catalyzed reaction at...

In Michaelis-Menten kinetics, what values would make an enzyme “good” at catalysis?

1. The assumptions made in calculating the Michaelis-Menten Equation include Question options: a) that the value...

1. The assumptions made in calculating the Michaelis-Menten Equation include Question options: a) that the value of k-2 can be ignored. b) that the concentration of the substrate is greater than the concentration of E. c) that the formation and decomposition of ES is the same for a period of time. d) A, B and C e) A and B only 2. Which of the following pairings regarding cofactors is correct? Question options: a) NADH and NADPH: contain isoalloxazine component...

When analyses enzyme-kinetics, what can we know with Michaelis-Menten equation? Write down everything you can.

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