Question

A new tumour suppressor gene (Tu015) has been identified which is associated with breast and ovarian cancer. Describe an experimental strategy using genetically modified mice to confirm that Tu015 is a tumour suppressor gene, rather than an oncogene, responsible for breast and ovarian tumours. Please explain briefly.

Answer 1

Genetically modified mice models using technology is available to control the overexpression and gene expression i.e done either through out mouse or in specific organ.Same method can be used in case to coform activity tumor suppression gene.With targeted mutagenesis scientists make specific models ,single gene defects can be readily mimicked in mice once associated gene is cloned.Mutation in inhibin gene appear to cause tumours in the ovaries;which shows that it is a potentil tumour suppressor gene.Eulopidy condition in case of Tu015 ,a cell or organisam has one or more than one complete set of chromosomes.Here a mice can be used to check the above condition by genetical modification .Tumor supperssor gene on adding to it there will be effect on their growth and functioning of cells ,whose loss of function leads to tumerogeneisis,which insights into mechanisam of human cancer.On specifically conducting on specific tissue types we can coform the Tu015 responsible foe breast and ovarian cancer. This cofirmation comes from identification of growth on tissues and loss of heterozygosity which occurs when Tu015 become inactivated ,when tumor suppressor gene mutation acquires.