Question

In: Biology

The constituents of a media for growing Hydrogenovibrio crunogenus are listed below. CONSTITUENTS &

The constituents of a media for growing Hydrogenovibrio crunogenus are listed below.

CONSTITUENTS ____ AMOUNTS

Sodium bicarbonate (NaHCO₃) 5.0 grams

Ammonium phosphate, monobasic [NH₄H₂PO₄] 1.0 gram

Sodium chloride [NaCl] 5.0 grams

Magnesium sulfate [MgSO₄ . 7H₂O] 0.2 gram

Potassium phosphate, dibasic [K₂HPO₄] 1.0 gram

Iron III carbonate [Fe₂(CO₃)₃] 5.0 grams

Water [H₂O] 982.8 grams

1. What type of microorganism is Hydrogenovibrio crunogenus based on the ingredients of this media-photoheterotroph, chemoautotroph, chemoheterotroph, or photoautotroph?

2. What are the sources of nitrogen and phosphorus in this media?

3. Is the media chemically-defined or complex?

4. Is the media a liquid or semi-solid?

Expert Solution

Answer-

According to the given question-

Hydrogenovibrio crunogenus - also called Thiomicrospira crunogena is a sulfur-oxidizing bacteria found in deep-sea hydrothermal vent and are colourless , and considered as obligate chemolithoautotrophic sulfur oxidizer bacteria.

Here the media composition are

Sodium bicarbonate or NaHCO3 - 5.0 grams, Ammonium phosphate, monobasic or NH4H2PO4- 1.0 gram, Sodium chloride or NaCl - 5.0 gram , Magnesium sulfate or MgSO4 . 7H2O - 0.2 gram, Potassium phosphate, dibasic or K2HPO4 - 1.0 gram, Iron III carbonate or Fe2(CO3)3 - 5.0 grams , Water- 982.8 gm.

based on the ingredients of this media the microorganism Hydrogenovibrio crunogenus is considered as -, chemoautotroph, because they are synthesizing their energy from oxidizing inorganic compounds. Chemoautotrophic bacteria are present in extreme environments such as in ocean deep sea vents and are called extremophiles
sources of nitrogen - Ammonium phosphate, monobasic or NH4H2PO4 and source of phosphorus - Ammonium phosphate, monobasic or NH4H2PO4 , Potassium phosphate, dibasic or K2HPO4
This media is considered as chemically-defined media because in this media a definite amount and percentage of nutrient are fixed for the growth and development of microorganism.
This is a liquid media because here no agar was used which is used for solidifying the media.

gladiator answered 2 years ago

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