What will you mix together in your restriction enzyme digest to cut out the gfp gene?

What will you mix together in your restriction enzyme digest to cut out the gfp gene?

Expert Solution

Reaction mixture for digestion -

Genomic DNA containing GFP gene
Restriction enzyme with which we want to cut GFP gene
Buffer which will help in maintaining a constant pH for the working of restriction enzyme
Sterile water which will help in the making of the final volume of the reaction mixture

Restriction enzyme is an enzyme which is used to cut DNA at a specific location. It is also called as molecular scissors. It is a great importance in Recombinant DNA technology because it helps in the isolation of specific genes from the genomic DNA of an organism. It cuts DNA at a specific location called as restriction site which is a set of 4 to 8 base pairs. The enzymes are very specific for the restriction site. Even a single base pairs change in the restriction site unable the enzyme to cut the DNA.

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gladiator answered 2 years ago

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