In: Chemistry
make an energy level diagram (electronic transitions) of compound Fluorescein
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form ofluminescence. In most cases, the emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation. The most striking example of fluorescence occurs when the absorbed radiation is in the ultraviolet region of the spectrum, and thus invisible to the human eye, while the emitted light is in the visible region, which gives the fluorescent substance a distinct color that can only be seen when exposed to UV light. Fluorescent materials cease to glow immediately when the radiation source stops, unlike phosphorescence, where it continues to emit light for some time after.
Fluorescein is a synthetic organic compound available as a dark orange/red powder slightly soluble in water and alcohol. It is widely used as a fluorescent tracer for many applications.