Question

In: Biology

what is examples of DNA barcoding procedure? Examples of an experiment of a plant species DNA...

what is examples of DNA barcoding procedure?

Examples of an experiment of a plant species DNA barcoding? Add a breif explanation of the main steps?

What types of result would you get? what the result can tell you?
why barcoding the DNA of living beings?

Solutions

Expert Solution

Some examples of DNA barcoding procedures are : identifying plant leaves when flowers or fruits are not available, Identifying pollen collected on bodies of pollinating animals, identifying insect larvae which may have fewer diagnostic characters than adults. etc..

Identifying plant leaves in the absence of flowers and fruits, Identifying pollen collected on body of pollinating animals are two examples of experiment of a plant species DNA coding. There are two main steps : building the DNA barcode library of known species, and matching the DNA barcode sequence of an unknown sample against the DNA barcode library. The first steps requires taxonomists to select one to several individuals per species to serve as reference samples in the DNA barcode library. Tissue can be obtained from specimens already housed in hereria or can be taken directly from live specimens in the field with appropriate pressed, labelled and voucher specimens. These vouchers serve as critical permanent record that connects the DNA barcode to a particular species of plant. Once the DNA barcode library is complete for the organism under study, whether they compromise a geographical region, taxonomic group or target assemblage, then the DNA barcodes generated for the identified samples are compared to the known DNA barcodes using some type of matching algorithm.


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