Describe the components of a HPLC. How would you improve the accuracyof the analysis, if necessary?

Expert Solution

High Performance Liquid Chromatography or HPLC is a technique that enebles the components of a mixture to be segregated easily. It has the following components:

1. Column : it is a tube that contains beads that have affinity towards the components of the mixture. When the mixture of interest is passed through the column in a solvent, due to differences in the properties of components, they go through the column at different rates and get separated.

2. Pump : it pumps the solvent into the column at high pressure.

3. Injector : it injects the liquid sample in the solvent stream flowing inside the column.

4. Detector : it detects individual molecules that come out of the column.

5. Data system : it controls all parts and takes data from the detector and finds out the retention time and the amount of sample.

The accuracy can be increased in the following ways:

1. Packing the column with less diameter particles.

2. Increasing the accuracy of the detector and data System.

queen_honey_blossom answered 1 year ago

Describe the circumstances in which you would undertake a principle components analysis (PCA). What checks you...

Describe the circumstances in which you would undertake a principle components analysis (PCA). What checks you would undertake to assess the strength of the relationship among the variables before conducting a PCA?

Describe how ratio analysis can be used to improve operations and reduce debt ? Describe how...

Describe how ratio analysis can be used to improve operations and reduce debt ? Describe how identifying trends and patterns in financial statements over reporting periods can yield insights into the client’s business ?

10. How would you carry out the following analysis? Give all the necessary steps required and...

10. How would you carry out the following analysis? Give all the necessary steps required and also mention your choice of instruments. a. Hg contamination in a river b. Parts per trillion level of Pb in drinking water c. PAH in river water. d. Gasoline contamination in ground water.

Describe the historical aspects and methods for identifying underserved populations. Discuss how you would improve these...

Describe the historical aspects and methods for identifying underserved populations. Discuss how you would improve these methods, track a population's progression, and/or determine whether a population is no longer underserved.

7. How would you carry out the following analyses? Describe the necessary sample preparation steps and...

7. How would you carry out the following analyses? Describe the necessary sample preparation steps and your choice of appropriate analytical instrumentation: a. Simultaneous determination of Pb, Zn, Cr, and Cd in crops grown in soil treated with sewage sludge. b. Determination of pesticide residues in apples. c. Determination of Pb in human plasma. d. Determination of SO4-2, NO3-, and PO4-3 in compost to be used for lawn fertilizer. e. Screening for gasoline contamination around underground storage tanks. f. A...

Describe the necessary components of an infrastructure that supports goal deployment and continual improvement?

1a.In your own words describe how would you try to improve the following companies/organizations today and...

1a.In your own words describe how would you try to improve the following companies/organizations today and going forward. 1b. How do you believe that leaders can better communicate and demonstrate empathy and compassion on the job today and going forward?

Explain how a qualitative anatomical analysis can be applied to improve training technique. Why would a...

Explain how a qualitative anatomical analysis can be applied to improve training technique. Why would a clinician or practitioner want to do this and how could it help?

You are the manager of a retail store.Describe how you would improve the ability and effort...

You are the manager of a retail store.Describe how you would improve the ability and effort of workers by through each of the following: 1.recruiting 2.socializing 3.training 4.motivation 5.rewarding 6.evaluating 7.compensating

How could solubility of an analyte impact its results in HPLC? How would the peak differ,...

How could solubility of an analyte impact its results in HPLC? How would the peak differ, for example? How would a more soluble molecule's peak differ from a less soluble one? Also, what are the peak areas based on in HPLC? Why is one peak sharper and higher than another, for example? Does that relate to how well a molecule is retained by the stationary phase in the chromotography? If you change the detection wavelength, how would the peak area...

Question