What is inverse PCR? Describe it?

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Polymerase Chain reactions is a method used for amplifying the DNA using set of complementary primers that are specific for a sequence.When only one sequence of DNA is known and a Polymerase Chain reaction is done to amplify that DNA, then the reaction is said to be Inverse polymerase chain reaction or Inverse PCR.This is done by using primers that can amplify the unknown regions near the known regions on a DNA template.

Following is the principle of Inverse PCR Technique:

When a specific DNA region is known,then its sequence can be used to amplify the unkown flanking region of the inserted DNA.This amplification is done onto the cyclic enzymatic reaction with the help of primers that is specific to the known DNA sequence. On the outside of the known DNA region,they synthesis of DNA occurs.
When restriction digestion is done on the DNA genome, it digest only the unkown flanking region,leaving the known DNA region as it is.
Using a set of primers amplification of circular DNA can be done,which is generated by the ligation of flanking regions of the unknown DNA.

gladiator answered 1 year ago

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