1. How is PCR used to isolate a particular part of the
genome?
2. What is the purpose of a PCR primer?
3. Why is PCR used after isolating genomic DNA for the purpose
of examining a SNP?
As a reminder, PCR is the technique used to replicate DNA in
the lab. PCR is based on the way DNA is replicated in cells.
During PCR template DNA plus other ingredients are placed in a
test tube and the solution goes through repeated cycles of heating
and cooling.
The 'ingredients' of a basic PCR reaction are: DNA template,
primer, nucleotides, DNA polymerase, and any salts required for
polymerase function (buffer).
No ligase is required in PCR but it is...
Why do scientists do experiments using PCR? More specifically,
what are the temperatures used for PCR and what are the objectives
for each temperature?
Real time PCR: how is it analyzed? what can be done to improve
real time PCR? How do you know if the bullfight was good or not?
What does it mean if the real time parameters are high, low, or if
there were faults in something in the process seeing the results?
Abound in your response.
Real time pcr:
• How to do?
• How it is interpreted?
• How it is optimized