What is the purpose of PCR ?

Expert Solution

Polymerase chain reaction or PCR is a comparitively new method in molecular biology which is used for the amplification of DNA in fragment of gene form which was discoverd by Kary Mullis in 1981.

The DNA of extincting organisms, the rare and one time gettable genetic molecules havs to be preserved. The genentic information, if present in required amount has immense role in molecular biology.

PCR amplifies the DNA sequence and produce millions of its copies. The technique is now used in medicine, archeology, forensic, evolutionary biology, molecular biology etc fields.

PCR is a thermocycler which mimics the cellular conditions in a laboratory and the replication occurs in a large amount of time. The PCR machine setups the temparature and required molecules which triggers the process.

Incredients include the primer, dNTPs, DNA polymerase, the sample DNA etc..

The steps includes denaturation, annealing and extension. The denaturing temparature separates the strands, annealing temparature causes polymerization and extension of strand occurs.

The main purpose of PCR technique is the amplification of DNA particles.

gladiator answered 2 years ago

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