Researchers mixed two plates of cells, one being ovarian follicle granulosa cells and the second cardiac...

Researchers mixed two plates of cells, one being ovarian follicle granulosa cells and the second
cardiac muscle cells (which contract in response to norepinephrine). They noticed that the cardiac
cells responded to follicle stimulating hormone (FSH) by contracting, even though in unmixed plates
of cardiac muscle cells, there was no response. Answer the following 3 questions. (3 points)
A. What does FSH signaling produce to which cardiac cells may react? (1 pt)
__________________
B. How would this compound be transferred to the cardiac cells? (1 pt)
__________________
C. How could you test your answer experimentally in ‘b’? one sentence (1 point)

Expert Solution

A. It produces norepinephrine whose effects are mediated through interaction at ?-1 and ?-1 receptors. when it binds with ?-1 receptors in the myocardium (beta-adrenergic receptor which is a G-protein coupled receptor expressed predominantly in cardiac tissue) it initiate the beta-adrenoceptor adenylyl cyclase system which leads to protein phosphorylation of L type Ca channels which increase the Ca influx and cause activation of Ryanodine receptors resulting an increase in myocardial contractility and myocardial oxygen requirements.

B. Cardiac muscle cells are connected by regions called intercalated discs. Intercalated discs contain gap junctions and desmosomes. These gap junctions are protein-lined tunnels that allow direct transmission of the depolarizing current or even passage of small molecules from cell to cell.

C. add a gap junction blocker such as octanol or Mefloquine to the cardiac cell culture before mixing it with the granulosa cells and the check for the occurance of contractions after mixing.

gladiator answered 1 year ago

After ovulation, the granulosa cells from the ovulated follicle differentiate into luteal cells. Steroid production by...

After ovulation, the granulosa cells from the ovulated follicle differentiate into luteal cells. Steroid production by granulosa cells in a developing follicle is different from those forming the corpus luteum because: choose the correct answer secretion of hormones by the luteal cells has negative feedback effects on the hypothalamus and pituitary whereas secretion by the granulosa cells has only positive feedback effects. steroid hormone secretion by the granulosa cells mainly promotes the formation of secretory glands in the endometrium, whereas...

Two new mathematics learning techniques are being tested. The researchers are unsure which one, if any,...

Two new mathematics learning techniques are being tested. The researchers are unsure which one, if any, will be better. Two hundred students were randomly selected from a population. Ninety of them were randomly assigned to use Technique A, and 110 of them were randomly assigned to use Technique B. Each student spent 30 minutes learning the technique to which they were assigned, and then were asked to complete a task. The time to complete the task was recorded, in seconds....

Game theory: Analyze the second price" auction. There are two bidders and one object being sold...

Game theory: Analyze the second price" auction. There are two bidders and one object being sold by auction. Each bidder knows what the object is worth to him, but not what it is worth to the other bidder. In other words, the object is worth v1 to bidder 1 and v2 to bidder 2. Bidder 1 knows v1 but not v2, while bidder 2 knows v2 but not v1. In the sealed bid second price auction, each bidder privately submits...

A research had two identical plates of Hela cells. The researcher performed a trypan blue viability...

A research had two identical plates of Hela cells. The researcher performed a trypan blue viability test on the first plate. The second plate was transfected with a GFP expression plasmid. Six hours after the transfection the researcher observed the number of fluorescent cells and then performed a trypan blue viability test. The results are show below: Plate 1 Plate 2 Trypan Blue Test Blue 50 100 Clear 200 150 Fluorescence Test Green --- 75 ...

Both neurons and cardiac muscle cells have action potentials. Discuss at least two ways in which...

Both neurons and cardiac muscle cells have action potentials. Discuss at least two ways in which neural and cardiac action potentials, and the way they spread between cells, are different.Both neurons and cardiac muscle cells have action potentials. Discuss at least two ways in which neural and cardiac action potentials, and the way they spread between cells, are different.

Two flat plates of glass with parallel faces are on a table, one plate on the...

Two flat plates of glass with parallel faces are on a table, one plate on the other. Each plate is 11.0 cm long and has a refrac- tive index of 1.55. A very thin sheet of metal foil is inserted under the end of the upper plate to raise it slightly at that end, in a man- ner similar to that discussed in Example 35.4. When you view the glass plates from above with reflected white light, you observe that,...

Late one night, a patient being treated after cardiac arrest expresses to her family and health...

Late one night, a patient being treated after cardiac arrest expresses to her family and health care providers her desire to forego further resuscitation efforts. Her family does not agree with her decision. The health care providers believe that the patient is sufficiently competent to make her own choices. However, a second cardiac arrest occurs before legal action is taken. Discuss the issues involved when patients, their families, and health care providers disagree.

2 Example of these pairs: In myeloid cells, pair of cell types; one being a circulating...

2 Example of these pairs: In myeloid cells, pair of cell types; one being a circulating form and the other is similar, but a resident tissue version

A viscous incompressible fluid is between two parallel plates (that are along the x-z plane), one...

A viscous incompressible fluid is between two parallel plates (that are along the x-z plane), one of them is at y = 0 and the other at y = d. a) Considerthecasewheretheflowisstationaryinthezˆdirection, driven by a pressure gradient. Write down the Navier-Stokes equations and solve for the velocity field after imposing the relevant boundary conditions. [10 points] b) The pressure gradient is now removed, but the plate at y = d is moved in an oscillatory fashion with velocity ue−iωt in...

You are given two containers, the first containing one litre of wine and the second one...

You are given two containers, the first containing one litre of wine and the second one litre of water. You also have a cup which has a capacity of r litres, where 0 < r < 1. You fill the cup from the first container and transfer the content to the second container, stirring thoroughly afterwards. Next, dip the cup in the second container and transfer r litres of liquid back to the first container. This operation is repeated again...

Question