I discovered a new transcription activator (a protein that binds a specific DNA sequence at promoters...

I discovered a new transcription activator (a protein that binds a specific DNA sequence at promoters of genes) and helps bring in the SWI/SNF complex to the promoters of 200 genes. What would be the affect on chromatin organization and gene expression at these genes if I mutate the transcription activator such that it no longer is able to bind DNA?

Solutions

Expert Solution

Answer: SWI/SNF (SWItch/Sucrose Non-Fermentable) complex is ATP dependent chromatin remodeling complex that causes nucleosome rearrangements and allow RNA polymerase and other regulatory proteins to access the chromatin easily and binds to promoter, leading to expression of genes in that region. If a transcription activator that helps to recruit SWI/SNF complex to the promoter of 200 genes is mutated, the expression of those 200 genes does not occur. This is because SWI/SNF (SWItch/Sucrose Non-Fermentable) complex are not recruited to the promoter by mutated transcription activator and the complex is unable to bind to the promoter to cause nucleosome rearrangements, leading non-exposure of binding sites for RNA polymerase and other regulatory proteins on the chromatin. As the RNA polymerase and other regulatory proteins are not able to access the chromatin and binds to the promoter, the expression of 200 genes will not occur.

gladiator answered 1 year ago

Next > < Previous

Related Solutions

In isolation, a DNA-binding protein binds to its regulatory sequence with a Kd of 1.0 microM....

In isolation, a DNA-binding protein binds to its regulatory sequence with a Kd of 1.0 microM. Another DNA binding protein binds to another sequence on the same DNA a few bases away with a Kd of 5.0 microM when alone. The two proteins each have a domain which binds to the other, with an interaction energy of -2.7 kcal/mole: (a) Draw the thermodynamic box which represents all four states of this system. (b) what are the affinities for each protein...

You are studying a new protein called protein Z that you think is a gene-specific transcription...

You are studying a new protein called protein Z that you think is a gene-specific transcription factor that enhances expression of genes that are involved in metabolism. Using an EMSA, you have already shown that protein Z binds to enhancers of a group of genes that code for proteins involved in metabolism. In order for a gene-specific transcription factor to be involved in regulating transcription of a gene that may be located far away, what protein does the gene-specific transcription...

During eukaryotic transcription, TFIID recognizes and binds the ____ box in the DNA. The technique initially...

During eukaryotic transcription, TFIID recognizes and binds the ____ box in the DNA. The technique initially used in the identification of promoter sequences was ________________. A more contemporary technique for identification of promoter sequences is the _________ _____________________ assay.

The promoter is an important sequence for transcription of a gene. Which of these components binds to the promoter of a eukaryotic gene to allow transcription to begin?

The promoter is an important sequence for transcription of a gene. Which of these components binds to the promoter of a eukaryotic gene to allow transcription to begin? Select all that are correct.A. Ribosomal subunitsB. A start codonC. DNA polymerase enzymeD. General transcription factorsE. RNA polymerase enzyme

An ER signal sequence found at the N-terminus of a protein ____. binds to SRP is...

An ER signal sequence found at the N-terminus of a protein ____. binds to SRP is positioned within the ER membrane, with the N-termus facing the cytosol. is always cleaved by signal peptidase all of these answers

how is DNA changed during transcription? chemically? structurally? be specific

A new operon is discovered with 5 genes. How many promoters with a -10 and -35 region...

A new operon is discovered with 5 genes. How many promoters with a -10 and -35 region for sigma factor binding does it have? How many ribosome binding sites (Shine Delgarno sequences)? Explain your answer.

Assume you know or have access to the sequence of DNA or a protein unless the...

Assume you know or have access to the sequence of DNA or a protein unless the question states that its sequence is unknown. You are investigating an anti-cholesterol drug designed to lower LDL but not HDL. The drug interferes with lipid processing by blocking a conversion enzyme. Some reports from the clinical trials indicate that people with a SNP in the conversion enzyme gene sequence do not respond to the drug. Explain how could you develop an experimental model to...

1. Which DNA sequence is recognized first to initiate transcription in eukaryotes? A. TATA box B....

1. Which DNA sequence is recognized first to initiate transcription in eukaryotes? A. TATA box B. poly A sequence C. coding sequence D. enhancer 2. Strand initiation in DNA replication is accomplished when the enzyme primase lays down a primer. In prokaryotes, of what molecule is that primer composed? A. DNA B. RNA C. protein D. lipid

Identify the indicated features of the transcription initiation complex, if it is protein or DNA associated, and if it is cis- or trans-acting.

Identify the indicated features of the transcription initiation complex, if it is protein or DNA associated, and if it is cis- or trans-acting.LocationTranscription FactorProtein or DNACis- or trans-actingActivatorEnhancer elementInitiator elementMediatorTATA boxTFIID complexRNA polymerase

Subjects