Question

1. If you wanted to determine whether a gene was present in a mass of DNA, what would be the best tool to use to show whether that gene was present or not?

a) Plasmid.

b) Restriction enzyme.

c) Probe.

d) Micropipette.

e) Magnifying glass.

2. If you wanted to cut a strand of DNA, what would be the best tool to use?

a) Plasmid.

b) Restriction enzyme.

c) Probe.

d) Micropipette.

e) Magnifying glass.

3. Which of the following is not a current use of DNA fingerprinting?

a) Determining paternity of offspring.

b) Determining the innocence of suspects in criminal investigations.

c) Determining the origin and nature of hides, meat, and other types of contraband.

d) Doing population surveys from animal fur or feathers.

e) Mark e if all are potential uses of DNA fingerprinting.

4.Assume that one individual is homozygous for a VNTR, and his two homologous chromosomes have a sequence of nucleotides that repeat three times on each chromosome. Another individual is heterozygous for a VNTR. One of his homologous chromosomes has a sequence of nucleotides that repeats three times, while on the other homologous chromosome the sequence repeats four times. Which statement is true if a DNA fingerprint was done on each person, and one of the genes targeted in the fingerprint is theVNTR described above? In other words the fingerprint would generate RFLP’s that included thisVNTR.

a) The individuals would produce an identical banding pattern for the RFLP’s that contain thisVNTR.

b) The individuals would produce different banding patterns for the RFLP’s that contain thisVNTR.

5. If a researcher wants to cut a gene from a sample of DNA, and put that gene into a bacterial plasmid, what should that researcher do?

a) The researcher should use a restriction enzyme to cut out the gene of interest from the DNA

sample, and use the same restriction enzyme to make a cut in plasmids. Then mix the genes and

plasmids together allowing the gene of interest to reanneal within plasmids.

b) The researcher should use a restriction enzyme to cut out the gene of interest from the DNA

sample, and use a different restriction enzyme to make a cut in plasmids. Then mix the genes and plasmids together allowing the gene of interest to reanneal within plasmids.

6. When scientists incorporate a gene into a plasmid they include a marker gene. What is the purpose of the marker gene?

a) It causes the bacterium to divide forming a colony.

b) It produces a restriction enzyme.

c) It produces a dye or in some way distinguishes bacterial colonies that have incorporated the

gene, from those that have not.

7. Which statement is not true of gene cloning?

a) It is a process to produce multiple copies of a gene.

b) It is identical to the process known as PCR.

c) It uses bacteria to make copies of genes inserted into bacterial plasmids.

8. Which of the following is a technique to incorporate “foreign” DNA into a cell?

a) Viral carriers.

b) Direct injection.

c) Gene gun.

d) Mark d if all are techniques to incorporate “foreign” DNA into a cell.

9. Which statement is true of gel electrophoresis?

a) It separates fragments of DNA according to their length.

b) The shortest strands of DNA move the slowest, and the longest strands move the fastest.

c) It is a technique to introduce mutations into DNA.

d) It is a process which causes the DNA to make millions of copies of itself.

10. Which of the following is not required for PCR?

a) RNA primers.

b) Taq polymerase.

c) Restriction enzymes.

d) DNA nucleotides (adenine, guanine, cytosine, thymine).

11. Which of the following is the most difficult to clone?

a) Carrot.

b) Frog.

c) Chimpanzee.

12. Which statement is not true of stem cells?

a) They are derived from the stems of plants.

b) They have the potential to differentiate into many types of cells.

c) They have potential value in the treatment of diseases such as Parkinson’s Disease, Alzheimer’s

Disease, and Diabetes.

d) Embryonic stem cells are pluripotent, and the most useful in disease treatment.

13. The best way to separate a mixture of DNA molecules by size is to use

a) PCR

b) Gel Electrophoresis

c) Recombinant DNA techniques

d) restriction enzymes

e) Gene cloning

14. Which statement is not true of cloning of mammals, as it was done with the sheep, Dolly?

a) The nucleus of the ovum is removed and replaced with the diploid nucleus from an adult “donor”

cell.

b) An electrical pulse triggers cleavage and development of the egg—the egg behaves as if it had

been fertilized.

c) The scientific community has complete confidence in the safety of cloning, and is ready to begin

cloning humans.

d) The clone is genetically identical to the “donor” animal, i.e. the animal that “donated” the

nucleus.

15. A DNA fingerprint can be determined using:

a) Restriction enzymes and gel electrophoresis

b) Primers and PCR

c) Recombinant DNA Technology

d) Mark "d" is both a and b are true

e) Mark "e" is both a and c are true

16. Which of the following is not a method to introduce genes into cells

a) Transduction

b) Direct injection

c) Transformation

d) Gene gun

e) Primers

17. Why does DNA migrate towards an anode during gel electrophoresis?

a) Because the deoxyribose sugars in DNA have an overall positive charge.

b) Because DNA has an overall negative charge

c) Because DNA fragments can differ by size

d) Because RFLPs differ in length for each individual

e) Mark "e" if all the above are true.

18. If you wanted to target a section of DNA with the nucleotide sequence 3'CCGTA5' you probe that is

a) 3'CCGTA5'

b) 5'CCGTA3'

c) 5'ATGCC3'

d) 3'GGCAT5'

e) 5'GGCAT3'

19. During PCR, what determines which specific section of DNA will be replicated?

a) DNA (Taq) Polymerase

b) RNA primers

c) VNTRs

d) High heat during the denaturation step

e) RNA polymerase

20. The combined DNA of two or more different species is called

a) Gene cloning

b) Restriction endonucleases

c) Recombinant DNA

d) Plasmids

e) Stem cell DNA

Answer 1

1)c probe

dna probe can be used to isolate a particular gene from a mass of dna it is a hybridization level that can be radioactivly labelled.it can be used to detect sample s of complementary nucleotide sequence.

2)b restriction endonuclease

It is the best tool to cut a dna sample at excact specific recognition sites which are also known as recognition site the restriction endonuclease is an enzyme.

3)e all are potential use of dna fingerprinting.

All the techniques use dna fingerprinting it helps to determine the individual constituents of dna which donot match with others which helps them to analyse and identify.

4)b the individual would prOduce different banding patterns for rflp that contains this vntrs.

5)a

The resarcher should use a restriction enzyme to cut out the gene of interest from dna sample and use the same restriction enzyme to cut make a cut in plasmid and mix the gene and plasmid together because they will form pallindromic dna and rdna eill be formed.there bases will match.

6)c

It produces a dye or in some way it distinguishes a colony that has been incorporated from those that not.they are known as selectable marker s they help in determining particular colonies.

7)c it os not identical to pcr as

Gene cloning produces multiple copies of desired dna invivo and pcr produces invitro amplification of desired dna by repeated cycle s.

8)d

All the techniques are used to incorporate foreign dna into a desired dna sample

9)a according to their length

All fragments are separated according to their size during gel electrophoresis sometimes they are also separated according to their charge.

10)d dna nucleotide s

Pcr helps to add more nucleotide s so it does not require any nucleotide ot only reuires an primer which helps ot to repeat its cycle and produce more nucleotide sequence.

11)c chimpanzee

Cloning

Will be most difficult as it is most higher grade animal with more dna sequence so cloning of chimpanzee is difficult.

12) a they are not derived from plant

Stem cells have the capability to differentiate . they are found in multicellular organisms.they are derived from inner cell mass in embryos and in adults from tissues.

13)gel electrophoresis b

In gel electrophoresis a mixture of dna which are protein by nature dna which are separated by size and the amount of charge present in them.

14)c the scientific community does not havr any fate in cloning and human cloning is not allowed.

15)d both a and b are used in process of dna finger printing first primers and pcr help in increasing the number of dna and then gel electrophoresis helps in separating them.

16)e primers are not used to introduce genes into cell they qre used to u initiate a gene replication process.

17)b

Dna is overall negatively charged so it migrates towards anode which is overall positively charged.

18)e 5'GGCAT3' if its dna probe

19)b rna primers helps in determining which particular segment of dna to be replicated.

20)c recombinant dna

It is the combined dna of two or more species .it is known as recombinant dna.