Question

Pre-Lab Questions:

1. What does amylase degrade? How will we know if amylase is present?

2. What type of catabolism discussed in this lab does not require the presence of molecular oxygen (O₂)?

3. How many OF-glucose tubes will we be inoculating with each bacterial strain?

Answer 1

answer) amyalse is a digestive enzyme which help in the digestion of the starch and convet into simple sugars or glucose. the prsence and absence can be done with the benedicts  test in this test we can take 2 test tube and putting unknown solution in it if thhe colour changes to organ then it consist of simple sugars.

answer) anaerobic respiration and alcholic fermentation is the type of catabolism that doesnot requires the oxygen for catabolism and produces lest of ATP and lactic acid and ethanl as their by products.

answer) OF ( oxidative/ fermentation glucose test) is abiological technique utilizedd in microbiolog to determine the way a microorganism metabolizes a carbohydrate such as glucose.

to perform this test two OF test tubes are incoculated with the test organism. with a layer of mineral oil is added to the toop f the deep in one of the tubesto create anaerobic condition

if the medium tuns yellow then the bacteria is fermenting glucose and is it doesnot convert yellow then bacteria oxidising gluocse.