Question

I. Use the DNA sequence below, which encodes a prokaryotic gene to answer the following questions.

1 ATGAGGAGTT 11 GACACACAAG 21 AGGAGGTAGC 31 AGTATGGGTA 41 TAATCTAATG 51 CGTAATTGAG 61 GAGGTAGTTG 71 ACGTATGAAT 81 AGTTAACGTA 91 CGGGGGGGAA 101 ACCCCCCCTT 111 TTTTTTTTTC 121 GAGCAATAAA 131 AGGGTTACAG 141 ATTGCATGCT

b) What region of this prokaryotic DNA sequence will be transcribed into mRNA? Circle one. 1-131 71-119 74-149 54-119

c) What will the sequence be for the protein translated from this mRNA?

d) Where is the 3’UTR? Circle one. 9-131 41-119 81-141 87-119

II. Use the DNA sequence below, which encodes a eukaryotic gene to answer the following questions.

1 GGCGTATAAA 11 GCGACGACTG 21 TAGACTGATG 31 AGCCTATCCA 41 TGGACGCGCC 51 ATGGCCCTGT 61 AAGCGGTGCG 71 ATGCAATAAA 81 ACGCGTATCA 91 GTCATTCAGC 101 GTAGTCTGAT 111 GCCAGTCGAC 121 TGC

a) Write down the corresponding sequences, find them in the sequence above and label them:

TATA box sequence: _________________ (label as TATA)

Polyadenylation signal in corresponding mRNA: ____________ (label as Poly-A)

Kozak’ssequence in corresponding mRNA: ________________ (label as Kozak)

Start (initiation) codon in corresponding mRNA: _______ (label as start)

Stop (termination) codon in corresponding mRNA: _______ (label as stop)

b) What region of the mRNA contains the open reading frame that will be translated into protein? 41-101 27-35 51-59 71-77

c) Where is the 3’UTR? Circle one. 59-101 51-123 63-101 71-81  

Answer 1

1 ATGAGGAGTT 11 GACACACAAG 21 AGGAGGTAGC 31 AGTATGGGTA 41 TAATCTAATG 51 CGTAATTGAG 61 GAGGTAGTTG 71 ACGT ATG AAT 81 AGT TAA CGTA 91 CGGGGGGGAA 101 ACCCCCCCTT 111 TTTTTTTTTC 121 GAGCAATAAA 131 AGGGTTACAG 141 ATTGCATGCT

in the above-given gene sequence:

bold and capital - -35 sequence

bold (TATAAT)- -10 sequence (pribnow box)

italic (poly G, poly C)- termination hairpin loop

italicized underlined (poly T that corresponds to poly U in mRNA)- RNA releasing domain (termination)

b) What region of this prokaryotic DNA sequence will be transcribed into mRNA? 54-119

c) Shine-Dalgarno sequence (AGGAGG)- ribosome binding site - 59-64

AUG (ATG) start codon- 75th position.

UAA (TAA) stop codon- 84th position.

the region for the protein translated from this mRNA - 75-83

the sequence for the protein translated from this mRNA- Met- Asn- Ser

d) Where is the 3’UTR? 87-119

II.

1 GGCGTATAAA 11 GCGACGACTG 21 TAGACTGATG 31 AGCCTATCCA 41 TGGACGCGCC 51 ATG GCC CTG T 61 AAGCGGTGCG 71 ATGCAATAAA 81 ACGCGTATCA 91 GTCATTCAGC 101 GTAGTCTGAT 111 GCCAGTCGAC 121 TGC

a. TATA box sequence- TATAAA (5 - 10)

Polyadenylation signal in corresponding mRNA: AATAAA (75-80)

Kozak’ssequence in corresponding mRNA: CGC GCC AUG G (45-54)

Start (initiation) codon in corresponding mRNA: ATG (51-53)

Stop (termination) codon in corresponding mRNA: TAA (60-62)

b) What region of the mRNA contains the open reading frame that will be translated into protein? 51-59

c) Where is the 3’UTR? Circle one. 59-101 (59-80 is the UTR)