The following DNA sequence occurs in the nontemplate strand of a structural gene in a bacterium...

The following DNA sequence occurs in the nontemplate strand of a structural gene in a bacterium (the promoter sequence is located to the left but is not shown): 5’-GAATGTCAGAACTGCCATGCTTCATATGAATAGACCTCTAG-3’ Consider the ribonucleotide sequence of the mRNA molecule that is transcribed from this piece of DNA that you determined in the previous question. What is the amino acid sequence of the polypeptide encoded by this mRNA? You must show and explain all work/evidence to receive credit.

Solutions

Expert Solution

Given mRNA sequence

5’-GAA TGT CAG AAC TGC CAT GCT TCA TAT GAA TAG ACC TCT AG-3’

Anticodon of the mRNA sequence and it can obtained after translation

5’- CUU ACA GUC UUG ACG GUA CGA AGU AUA CUU AUC UGG AGA UC-3’

Amino acid corresponding to the anticodon after translation. It can be find by grouping 3 codons together. Each triplet of group of 3 base pairs code for one amino acid.

5’- leu thr val leu thr val arg ser ile leu ile trp arg-3’

(UC do not code for amino acid bcz it is 2 base pairs only)

leu- tryptophan- W

thr- Threonine -T

val- Valine- V

arg- Arginine- R

ser- serine- S

ile- Isoleucine- I

Explanation:

DNA- Guanine (G) always pair with the Cytosine (C) and Adenine (A) always pair with the Thymine (T)

RNA- Guanine (G) always pair with the Cytosine (C) and Adenine (A) always pair with the Uracil (U)

Codons- triplet of the nucleotide which code for on amino acid

DNA to mRNA : complementary pairing takes place

mRNA to polypeptide or protein or amino acids : complementary pairing takes place

gladiator answered 1 year ago

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