In: Chemistry
in urinalysis when testing for sugar why would sugar give a positive or negative result
Benedict's test
positive for reducing sugars
negative of r non reducing sugars
it can be explained as
Glucose was positive for reducing sugars, it demonstrated by the orange-red arrangement with a dim red hasten development situated at the base of the test tube. This exceptional shading infers the high centralization of lessening sugar present and accessible aldehyde assembles in the answer for diminish the copper (II) particles to frame the dim red hasten.
Maltose likewise tried positive for decreasing sugars with the substance change to an orange shading arrangement with dull red hasten. Lactose tried positive for decreasing sugars with consequences of a chestnut arrangement with dim red hasten. Maltose and lactose, both disaccharides, were relied upon to oxidize with Benedict's answer as they are both halfway made out of glucose which has an accessible aldehyde gathering to diminish the cupric particles and frame an encourage. Next, nectar shaped a chestnut arrangement with dull cocoa hasten inferring decreasing sugars are available in nectar. Nectar responds with Benedict's answer since fructose can be found in nectar giving a monosaccharide that gives a free aldehyde amass ready to respond with Benedict's solution
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Glucose-1-phosphate had no response with Benedict's solution , remaining a light blue arrangement with no accelerate. The compound structure of glucose-1-phosphate basically does not have an accessible aldehyde gathering to lessen cupric particles and as an outcome no response can happen. Sucrose was additionally inert with Benedict's answer, as there was no concoction change showed by either the nearness of encourage or by shading change. Sucrose in spite of the fact that contains fructose and glucose can't shape the aldehyde utilitarian gathering in light of its glycosidic bond . Thusly sucrose can't respond with Benedict's answer. In this test glycogen tried negative for diminishing sugars, it remained a light blue arrangement with no accelerate.
Glycogen, a polysaccharide, is a non-decreasing sugar proposing their shut ring structures make it hard to be separate. Starch like glycogen is a polysaccharide, thusly a nonreducing.