Question

In: Biology

matching fragment DNA at specific sites hybridize DNA fragments with labeled probe create DNA features on...

matching

fragment DNA at specific sites

hybridize DNA fragments with labeled probe

create DNA features on a DNA microchip

prepare mRNA for DNA sequencing

transform eukaryotic cells with electricity

detect the amount of or the 5’ end of mRNA

visualize DNA, RNA or other small structures

a type of electrophoresis

detect protein binding to DNA

use antibody to detect a specific protein

use antibody to purify a specific protein

amplify a specific locus of DNA

adding transformed ES to blastocyst

Type of DNA chip that looks at DNA with modified histones

A. electroporation

B. primer extension

C. PCR

D. electron microscopy

E. Southern blot

F. immunoprecipitation

G. photolithography

H. gel mobility shift assay

I. making transgenic mice

J. restriction enzyme digestion

K. Western blot

L. PAGE

M. ChIP

N. cDNA synthesis

O. microinjection

P. spotted microarray

Solutions

Expert Solution

as per the information given in the question, following is matching

A. electroporation - transform eukaryotic cells with electricity

B. primer extension - prepare mRNA for DNA sequencing

C. PCR - amplify a specific locus of DNA

D. electron microscopy -   visualize DNA, RNA or other small structures

E. Southern blot - hybridize DNA fragments with labeled probe

F. immunoprecipitation - use antibody to purify a specific protein

G. photolithography - create DNA features on a DNA microchip

H. gel mobility shift assay – detect protein binding to DNA

I. making transgenic mice - adding transformed ES to blastocyst

J. restriction enzyme digestion - fragment DNA at specific sites

K. Western blot - use antibody to detect a specific protein

L. PAGE - a type of electrophoresis

M. ChIP - Type of DNA chip that looks at DNA with modified histones

N. cDNA synthesis - detect the amount of or the 5’ end of mRNA

O. microinjection - adding transformed ES to blastocyst

P. spotted microarray - create DNA features on a DNA microchip


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