What kinds of sources can RNA be extracted from? What role(s) does the Guanidinium isothiocyanate play in RNA extraction?

What kinds of sources can RNA be extracted from? What role(s) does the Guanidinium isothiocyanate play in RNA extraction? What phase does the RNA go into after the addition of chloroform?

Expert Solution

RNA can be extracted from Animal cells, Animal tissue, Viruses, Plant tissue and bacterial cells and other microorganisms.

Guanidinium isothiocyanate is chaotropic agents it lyses the cells and denatures the proteins which bind to nucleic acid and also RNAses to protect the RNA.

After Addition of chloroform under the acid condition, RNA goes in upper aqueous phase from where it can be collected and precipitated by isopropanol.

gladiator answered 2 years ago

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