In: Biology
Please answer the whole questions
• Prokaryotic Initiation of Replication: formation of replication forks and start of polymerization
o What are the names of the binding sites contained in the replication origin and what proteins do they bind?
o What role does the enzyme Dam methylase play in the initiating replication?
o Describe the roles of DnaA, DnaB, DnaC•ATP, and Hu proteins in the initiation of prokaryotic replication.
o What is the DUE region in the replication origin and what role does it play in initiation of prokaryotic replication?
o Is primase (DnaG) required for the initiation of replication?
▪ What role does primase play in replication? Is that role necessary for replication to begin?
o What is the function of DNA Gyrase?
o Put all of the above together and tell the story of the initiation of prokaryotic replication.