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Please answer the whole questions • Prokaryotic Initiation of Replication: formation of replication forks and start...

Please answer the whole questions

• Prokaryotic Initiation of Replication: formation of replication forks and start of polymerization

o What are the names of the binding sites contained in the replication origin and what proteins do they bind?

o What role does the enzyme Dam methylase play in the initiating replication?

o Describe the roles of DnaA, DnaB, DnaC•ATP, and Hu proteins in the initiation of prokaryotic replication.

o What is the DUE region in the replication origin and what role does it play in initiation of prokaryotic replication?

o Is primase (DnaG) required for the initiation of replication?

▪ What role does primase play in replication? Is that role necessary for replication to begin?

o What is the function of DNA Gyrase?

o Put all of the above together and tell the story of the initiation of prokaryotic replication.

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