You have identified a mutation in a gene that results in enhanced drought resistance in tomato....

You have identified a mutation in a gene that results in enhanced drought resistance in tomato. It appears to encode a transporter. What will you have to do to determine whether the protein is associated with passive, active, or secondary active transport?

Expert Solution

The gene is known to encode for a transporter protein. This protein is present in the membrane. The transport can take place in one of the three ways, namely, passive transport, active transport or secondary active transport.

Active transport requires energy in the form of ATP. Hence, if an ATP synthesis blocker is added and then the transport across the protein is studied, it should be inhibited if it were an active transporter.

The secondary active transport uses the concentration gradient of some ions. It uses this gradient to facilitate the transport of the desired molecule. To check for this, the transporter protein can be embedded in a liposome that does not have a concentration gradient of ions. If the transporter fails to function here, then it is a secondary active transporter.

However, if the transporter works in both the above conditions, it is a passive transporter and does not require any external energy to function.

gladiator answered 2 years ago

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