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In tomato, mutants have been identified that do not produce any trichomes in contrast to wild...

In tomato, mutants have been identified that do not produce any trichomes in contrast to wild type plants. The transcriptomes (that means the nucleotide sequences of all mRNAs and small RNAs) of the wild type and mutant plants have been obtained. Describe how you could use this knowledge to determine possible genes that are responsible for the development of trichomes in the tomato leaf and stem epidermis?  

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Transcriptomes shows sequences of all the mRNA and small RNA present in a genome. So if we have the transcriptome profile of wild type and mutant tomato plant, we will get all the information related to mRNAs and small RNAs in this two scenarios. Now, transcriptome gives information about the amount of mRNAs and small RNAs of each gene which are expressed within a genome. It will show which sets of genes are highly expressed, or which set of genes are not expressed at all or the relative amount of different gene expression in these two plants. Now, in the mutant plant there is no ricome present, or the genes which are responsible for tricome formation are either expressed at low levels or not expressed at all. So if we look for the sets of genes which are present in wild type but either absent or expressed at low levels at mutant, we can identify the genes which will be responsible for trichome production.

gladiator answered 2 years ago
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