In: Biology
Which type of gene therapy results in alteration of the DNA of a gamete or a fertilized ovum?
Somatic gene therapy |
Ex vivo gene therapy |
In situ gene therapy |
Reversible gene therapy |
Germline gene therapy |
In a DNA microarray experiment, fluorescently labeled ______ are allowed to bind to known ______ sequences on a grid.
RNA; DNA |
DNA; RNA |
RNA; cDNA |
RNA; RNA |
cDNA; DNA |
RNA interference (RNAi) functions to:
turn on certain genes |
turn off certain genes |
prevent the translation of certain mRNA sequences |
prevent fertilization |
cause mutations in a specific part of the genome |
When a couple seeks information concerning the cause of their infertility, which member of the couple is usually tested first?
male |
female |
1. germline therapy
In this DNA is altered of the ovum or sperm or zygote itself.
Ex-vivo gene therapy gene is corrected outside the body.
Somatic gene therapy is done in somatic cells.
2. cDNA, DNA
DNA is present on the microarray and cDNA or DNA is used as a probe.
A glass slide or chip labeled with a population of specific DNA molecules
-The identity and specific location of each molecule is known.
-Fluorescently labeled DNA or cDNA probes are combined with microarray
-Unbound probes are washed away
-Location and intensity of fluorescent signal is read by laser scanner
-Intensity of signal is proportional to number of DNA probes bound to location on microarray.
3. prevent the translation of certain mRNA sequences
RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression or translation, by neutralizing targeted mRNA molecules.
4. Male
first test is semen analysis.