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In: Chemistry

explanation of column chromatography. Why do the compounds separate?

explanation of column chromatography. Why do the compounds separate?

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Expert Solution

Column Chromatography:

Column chromatography is one of the most useful methods for the separation and purification of both solids and liquids. This is a solid - liquid technique in which the stationary phase is a solid & mobile phase is a liquid. The principle of column chromatography is based on differential adsorption of substance by the adsorbent.

The usual adsorbents employed in column chromatography are silica, alumina, calcium carbonate, calcium phosphate, magnesia, starch, etc. selection of solvent is based on the nature of both the solvent and the adsorbent. The rate at which the components of a mixture are separated depends on the activity of the adsorbent and polarity of the solvent.

If the activity of the adsorbent is very high and polarity of the solvent is very low, then the separation is very slow but gives a good separation. On the other hand, if the activity of adsorbent is low and polarity of the solvent is high the separation is rapid but gives only a poor separation, i.e., the components separated are not 100% pure.

Suppose you wanted to separate a mixture of two coloured compounds - one yellow, one blue. The mixture looks green. then we use column chromatography.

The blue compound is obviously more polar than the yellow one - it perhaps even has the ability to hydrogen bond. You can tell this because the blue compound doesn't travel through the column very quickly. That means that it must adsorb more strongly to the silica gel or alumina than the yellow one.


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