Question

9. What do we do in our lab that corresponds to a fed batch culture?

1. Trypsinize and passage cells
2. Replace the medium as cells proliferate
3. Coat the plate with laminin
4. Plate cells, treat with an agent, and analyze
5. Vary the concentration of serum

10. What do we do in our lab that corresponds to a continuous culture?

1. Trypsinize and passage cells
2. Replace the medium as cells proliferate
3. Coat the plate with laminin
4. Plate cells, treat with an agent, and analyze
5. Vary the concentration of serum

11. What is the primary usefulness of solid microbeads in bioreactors?

1. Increase viscosity of the medium
2. Reduce agitation-induced damage to cells
3. Increase the surface area for adherent cells
4. Maximize dispersion of cells

12. What is the main goal of bioreactor approaches?

1. Optimize cell growth
2. Reduce medium requirements
3. Maximize product yield
4. Eliminate the need for serum

Answer 1

Ans. 9

Fed batch culture is a culturing method in which once a culture grown products remain in culturing vessel till the end. Option D is correct. Once a plate has been grown and after the introduction of agent nothing is added and removed from the plate and only analyzed for the affect of agent.

Ans. 10

Continuous culture is a culture method in which fresh media is added continually according to nutritional requirements and also wastes and products are removed from the culture vessel. Option B is correct.

Ans. 11

Microbeads are added in bioreacter to increase the required surface area for adhesion of the cells. It provides the attachment of cells in better way and efficiency of bioreacter is increased. Option C is correct.

Ans. 12

Optimisation is prime goal for operation of bioreacter. As in natural environment conditions may not be controlled in bertter way but in bioreacter optimum conditions may be provided for rapid growth of cultures. Option A is correct.