-What are Serial dilutions? how to do one, why we do one IN THE LAB and...

-What are Serial dilutions?

how to do one, why we do one IN THE LAB and the proper procedure.

-EXPLAIN what a titer is and how to determine it.

-How to determine the concentration based on the dilution.

Expert Solution

queen_honey_blossom answered 1 year ago

Explain how do we construct a standard curve in the UV/Vis spectrometer lab,what do we use...

Explain how do we construct a standard curve in the UV/Vis spectrometer lab,what do we use the standard curve for?

You perform serial dilutions to a dilution factor of 1,000,000 and aliquot 10uL on each media...

You perform serial dilutions to a dilution factor of 1,000,000 and aliquot 10uL on each media plate. The following table indicates the number of colonies on each plate at each dilution Dilution and # colonies neat TNTC (too numerous to count) 1:10 TNTC 1:100 TNTC 1:1000 TNTC 1:10,000 367 1:100,000 33 1:1,000,000 2 What is the concentration of the neat sample?

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What are the ledgers, why do we use them? And then HOW do we use them,...

What are the ledgers, why do we use them? And then HOW do we use them, how does information get into them how do balances get extracted. And then what should the balances for various accounts be, i.e. assets, liabilities, expenses, revenues, equity, dividends. Why SHOULD they have a particular balance as either debit or credit.

Intuitively, what is the discount rate, why do we need it, and how do we choose...

Intuitively, what is the discount rate, why do we need it, and how do we choose the appropriate number?

Your boss gives you an experimental antibiotic and wants you to make some serial dilutions of...

Your boss gives you an experimental antibiotic and wants you to make some serial dilutions of it to determine the MIC of the reagent on E. coli. If the initial concentration is 100 mg/ml and your boss wants you to make ten 2-fold serial dilutions of it, what will the concentration of the seventh tube in the series be (pay attention to units!)? a. 781.25 ug/ml b. 0.78125 ug/ml c. 3.125 mg/ml d. 0.00001 mg/ml

You are counting plaques on your plaque assay plates made from serial dilutions of your high...

You are counting plaques on your plaque assay plates made from serial dilutions of your high titer lysate. Your 10-5 plate has 615 plaques although some are butting up against each other so it is difficult to get an accurate count. Your 10-6 plate has 42 plaques, and your 10-7 plate has only 1 plaque. Which plate would probably yield the most accurate titer calculation of your phage and why is it more trustworthy than the others?

You are given the following 10-5 plaque counts from three different serial dilutions (a, b and...

You are given the following 10-5 plaque counts from three different serial dilutions (a, b and c). For each, fill in the table to show how many plaques you would estimate you would find on the plates with the dilution factors listed (10-6, 10-7, etc.) AND identify the dilution (ranging from 10-5 to 10-9)where theoretically the results should become untrustworthy (too few to count for a reliable titer calculation). 10-5 plaque count 10-6 10-7 10-8 10-9 55 5...

The cell density of the original broth was ‘x’ bacteria/ml. After two serial 1:100 dilutions and...

The cell density of the original broth was ‘x’ bacteria/ml. After two serial 1:100 dilutions and two 1:10 dilutions, the final spread plate has 25 distinct colonies. Estimate the cell density ‘x’ of the original broth.

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