Question

Explain what PCR is (p. 205) (Tablet p.233)
b. Explain the role of primers, dNTPs, Taq polymerase and buffer in the reaction
(Practical)

Answer 1

pcr is polymerase chain reaction. It is a technique that is used to amplify single copy or few copies of segment of DNA.

There are three steps involved i.e denaturatin - denature the DNA, annealing - ,cools the temperature that helps the primer to bind to the complementary sequence of single strand DNA, extention - raise the temperature and taq polymerise helps primer to extend.

role of primer - these are short pieces of single stranded DNA usually 20 nucleotides in length. There are two primers, they are designed so that they flank the targeted region of DNA. That is they are given sequence that makes them to bind to opposite strand of template DNA. It binds to complementary base pairing.

dntp - these are building blocks. These are monomeric blocks

taq polymerase - thermostable DNA polymerise. It attaches nucleotide to the DNA template thereby coping the dna

buffer - balances the ph . It creates an optimal condition for activity of taq dna polymerase.